Steve - it all depends on the speakers, there are no hard and fast rules. But GENERALLY speaking, tweeters aimed at your ears. I set my Bosch laser measurer on top of the speaker, in the middle of the speaker, get it flush with the front and aim it at my listening position. Gives me a laser beam to the right spot on the chair where my ears are. You would be surprised when you think you have it dialed in (by just eyeing it - looking at the speakers) - you don't! The laser is invaluable. I would say getting the tweeters lined up properly at your ears is critical to producing a properly centered image whereby vocals are realistic (meaning, with eyes closed, the singers voice appears to be a mouth width - and not 8 feet wide!).
I like to use a few different songs for this: Linvingston Taylor - Isn't She Lovely (the first 60 seconds where he is whistling) and Celine Dion - What a Wonderful World from her Miracle CD. She does the first part of the song a cappella.